low molecular weight poly i c Search Results


free low molecular weight poly i:c  (Dalton Pharma)
90
Dalton Pharma free low molecular weight poly i:c
Free Low Molecular Weight Poly I:C, supplied by Dalton Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+molecular+weight+poly+i+c/low+molecular+weight+poly+i+c/pmc09573954-290-17-18
Average 90 stars, based on 1 article reviews
free low molecular weight poly i:c - by Bioz Stars, 2026-09
90/100 stars
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high molecular weight (hmw) poly(i:c  (Enzo Biochem)
90
Enzo Biochem high molecular weight (hmw) poly(i:c
Zebrafish and human LGP2s play antithetic roles in regulating IFN response by poly(I:C) in fish cells (A–C) DrLGP2 and HsLGP2 downregulated fish IFN and human IFN promoter activation by poly(I:C) at a high concentration of 1 μg/ml in EPC cells. EPC cells seeded in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (A, B) , or HsIFNβpro-luc (200 ng each) (C) , together with DrLGP2 or HsLGP2 at increasing doses (0, 10, 50, 100, 200 ng). 24 h later, cells were transfected with 1 μg/ml poly(I:C) for another 24 h, followed by luciferase assays. P values were calculated using ANOVA. ** P < 0.01, *P < 0.05. (D–G) DrLGP2 and HsLGP2 switched a first positive role to a following negative one in regulating IFN response by increasing concentrations of poly(I:C) in EPC cells. EPC cells seeded overnight in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (D, E) , or HsIFNβpro-luc (F, G) , together with DrLGP2 (D, F) or HsLGP2 (E, G) (200 ng each). 24 h later, cells were transfected with poly(I:C) at increasing doses for another 24 h, followed by luciferase assays. P values were calculated using Student’s t-test. ** P <0.01. (H) Agarose electrophoresis showed the molecular weight spanning <t>of</t> <t>MMW</t> poly(I:C) and <t>HMW</t> poly(I:C). (I) RNA pull-down assays verified the binding of poly(I:C) to DrLGP2 and HsLGP2 in fish cells. CO cells seeded in 10 cm dishes were transfected with DrLGP2-HA, HsLGP2-HA or GFP-HA as control. 24 h later, cells were lysed. One-tenth of cell lysates were taken as input, the remaining was incubated with 100 ng biotinylated poly(I:C), followed by western blots with anti-HA antibody.
High Molecular Weight (Hmw) Poly(i:C, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+molecular+weight+poly+i+c/high+molecular+weight++hmw++poly+i+c/pmc09428467-38-16-21
Average 90 stars, based on 1 article reviews
high molecular weight (hmw) poly(i:c - by Bioz Stars, 2026-09
90/100 stars
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Zebrafish and human LGP2s play antithetic roles in regulating IFN response by poly(I:C) in fish cells (A–C) DrLGP2 and HsLGP2 downregulated fish IFN and human IFN promoter activation by poly(I:C) at a high concentration of 1 μg/ml in EPC cells. EPC cells seeded in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (A, B) , or HsIFNβpro-luc (200 ng each) (C) , together with DrLGP2 or HsLGP2 at increasing doses (0, 10, 50, 100, 200 ng). 24 h later, cells were transfected with 1 μg/ml poly(I:C) for another 24 h, followed by luciferase assays. P values were calculated using ANOVA. ** P < 0.01, *P < 0.05. (D–G) DrLGP2 and HsLGP2 switched a first positive role to a following negative one in regulating IFN response by increasing concentrations of poly(I:C) in EPC cells. EPC cells seeded overnight in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (D, E) , or HsIFNβpro-luc (F, G) , together with DrLGP2 (D, F) or HsLGP2 (E, G) (200 ng each). 24 h later, cells were transfected with poly(I:C) at increasing doses for another 24 h, followed by luciferase assays. P values were calculated using Student’s t-test. ** P <0.01. (H) Agarose electrophoresis showed the molecular weight spanning of MMW poly(I:C) and HMW poly(I:C). (I) RNA pull-down assays verified the binding of poly(I:C) to DrLGP2 and HsLGP2 in fish cells. CO cells seeded in 10 cm dishes were transfected with DrLGP2-HA, HsLGP2-HA or GFP-HA as control. 24 h later, cells were lysed. One-tenth of cell lysates were taken as input, the remaining was incubated with 100 ng biotinylated poly(I:C), followed by western blots with anti-HA antibody.

Journal: Frontiers in Immunology

Article Title: Function conservation and disparities of zebrafish and human LGP2 genes in fish and mammalian cells responsive to poly(I:C)

doi: 10.3389/fimmu.2022.985792

Figure Lengend Snippet: Zebrafish and human LGP2s play antithetic roles in regulating IFN response by poly(I:C) in fish cells (A–C) DrLGP2 and HsLGP2 downregulated fish IFN and human IFN promoter activation by poly(I:C) at a high concentration of 1 μg/ml in EPC cells. EPC cells seeded in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (A, B) , or HsIFNβpro-luc (200 ng each) (C) , together with DrLGP2 or HsLGP2 at increasing doses (0, 10, 50, 100, 200 ng). 24 h later, cells were transfected with 1 μg/ml poly(I:C) for another 24 h, followed by luciferase assays. P values were calculated using ANOVA. ** P < 0.01, *P < 0.05. (D–G) DrLGP2 and HsLGP2 switched a first positive role to a following negative one in regulating IFN response by increasing concentrations of poly(I:C) in EPC cells. EPC cells seeded overnight in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (D, E) , or HsIFNβpro-luc (F, G) , together with DrLGP2 (D, F) or HsLGP2 (E, G) (200 ng each). 24 h later, cells were transfected with poly(I:C) at increasing doses for another 24 h, followed by luciferase assays. P values were calculated using Student’s t-test. ** P <0.01. (H) Agarose electrophoresis showed the molecular weight spanning of MMW poly(I:C) and HMW poly(I:C). (I) RNA pull-down assays verified the binding of poly(I:C) to DrLGP2 and HsLGP2 in fish cells. CO cells seeded in 10 cm dishes were transfected with DrLGP2-HA, HsLGP2-HA or GFP-HA as control. 24 h later, cells were lysed. One-tenth of cell lysates were taken as input, the remaining was incubated with 100 ng biotinylated poly(I:C), followed by western blots with anti-HA antibody.

Article Snippet: The medium molecular weight (MMW) poly(I:C) was purchased from SIGMA (Catalog no. I3036), and the high molecular weight (HMW) poly(I:C) from Enzo Life Sciences (Catalog no. ALX-746-021).

Techniques: Activation Assay, Concentration Assay, Transfection, Luciferase, Electrophoresis, Molecular Weight, Binding Assay, Incubation, Western Blot

Zebrafish and human LGP2s play antithetic roles under low concentrations of poly(I:C) in mammalian cells and do so alone in fish cells. (A–E) Zebrafish and human LGP2s played antithetic roles under low concentrations of poly(I:C) in mammalian cells. HEK293T cells seeded in 24-wells plates were co-transfected with HsIFNβpro-luc (200ng), together with HsLGP2 (A–D) or DrLGP2 (E) (200 ng each). 24h later, cells were transfected again with MMW poly(I:C) [indicated as poly(I:C) in the text or all Figures] at 2 μg/ml (B, E) or at 4 ng/ml (C) , or with HMW poly(I:C) at 4 ng/ml (D) . Renilla vector (pRL-TK, 0.2 ng) was transfected as internal control. Another 24 h later, cells were collected for luciferase assays. P values were calculated using ANOVA. ** P < 0.01. (F) Overexpression of zebrafish or human LGP2s alone revealed antithetic roles in mammalian cells. EPC cells seeded in 24-well plates were transfected with DrLGP2 at the indicated increasing doses for 48 h. Or at 24 h post transfection, cells were transfected again with poly(I:C) at a high concentration of 1 μg/ml for another 24 h, followed by luciferase assays. P values were calculated using ANOVA. ** P < 0.01.

Journal: Frontiers in Immunology

Article Title: Function conservation and disparities of zebrafish and human LGP2 genes in fish and mammalian cells responsive to poly(I:C)

doi: 10.3389/fimmu.2022.985792

Figure Lengend Snippet: Zebrafish and human LGP2s play antithetic roles under low concentrations of poly(I:C) in mammalian cells and do so alone in fish cells. (A–E) Zebrafish and human LGP2s played antithetic roles under low concentrations of poly(I:C) in mammalian cells. HEK293T cells seeded in 24-wells plates were co-transfected with HsIFNβpro-luc (200ng), together with HsLGP2 (A–D) or DrLGP2 (E) (200 ng each). 24h later, cells were transfected again with MMW poly(I:C) [indicated as poly(I:C) in the text or all Figures] at 2 μg/ml (B, E) or at 4 ng/ml (C) , or with HMW poly(I:C) at 4 ng/ml (D) . Renilla vector (pRL-TK, 0.2 ng) was transfected as internal control. Another 24 h later, cells were collected for luciferase assays. P values were calculated using ANOVA. ** P < 0.01. (F) Overexpression of zebrafish or human LGP2s alone revealed antithetic roles in mammalian cells. EPC cells seeded in 24-well plates were transfected with DrLGP2 at the indicated increasing doses for 48 h. Or at 24 h post transfection, cells were transfected again with poly(I:C) at a high concentration of 1 μg/ml for another 24 h, followed by luciferase assays. P values were calculated using ANOVA. ** P < 0.01.

Article Snippet: The medium molecular weight (MMW) poly(I:C) was purchased from SIGMA (Catalog no. I3036), and the high molecular weight (HMW) poly(I:C) from Enzo Life Sciences (Catalog no. ALX-746-021).

Techniques: Transfection, Plasmid Preparation, Luciferase, Over Expression, Concentration Assay